Coding

Part:BBa_K357005:Design

Designed by: Elena Pasko   Group: iGEM10_Toronto   (2010-06-28)

3-oxoadipate CoA-transferase, subunit A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was optimized for E.Coli. A NgoMIV restriction site was identified around 277bp.

Source

SOURCE Pseudomonas putida KT2440

ORGANISM  Pseudomonas putida KT2440
          Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
          Pseudomonadaceae; Pseudomonas.

REFERENCE 1 (bases 1 to 696)

AUTHORS   Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,
          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,
          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,
          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,
          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,
          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,
          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,
          Duesterhoft,A., Tummler,B. and Fraser,C.
TITLE     Complete genome sequence and comparative analysis of the
          metabolically versatile Pseudomonas putida KT2440
JOURNAL   Environ. Microbiol. 4 (12), 799-808 (2002)


References